Cheek cells room eukaryoticcells (cells the contain a nucleus and other organelles in ~ enclosed in amembrane) that room easily melted from the mouth lining. It"s thus easy toobtain them for observation.
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Some the the key parts of acell include:
1. Cell membrane (outer boundary of the cell)
2. Cytoplasm (the fluid within the cell)
3. Nucleus (at the facility of the cell and controls cabinet functions)
4. Organelles (e.g. Mitochondria-Organelles are cell structures withspecific functions)
Using organic stainssuch together methylene blue, it"s feasible to clearly observe and also differentiate thedifferent components of a cell. This is due to the fact that the stain will shade some components ofthe cell and not others, permitting them to be clearly observed.
RequirementsSterile cotton swabMicroscope covering slipsMethylene Blue equipment (0.5% come 1%)DropperBlotting paper/Tissue paper
How to Prepare a Wet mountain of Cheek Cells
Before starting, it"salways important to ensure the the working surface is clean and also that girlfriend arewearing a pair the clean gloves to protect against contamination.
Cheek cells have the right to beeasily derived by tenderness scraping the within of the mouth making use of a clean,sterile cotton swab.
Once the cells have been obtained,the following procedure is used for cheek cell wet mount preparation:
place a drop of physiological saline ~ above a clean microscopic slide(central component of the slide)smear the noodle swab on to the facility (part include the salinedrop) the the clean on slide for around 4 seconds to gain the cell on come the centerof the slideadd a autumn of methylene blue solution on come the smear and also gentlyplace a cover on slide on top (to cover the stain and the cells)any excess solution have the right to be gotten rid of by emotional one side of theslide v a record towel or blotting paper.place the on slide on the microscopic lense for monitoring using 4 x or 10x target to find the cellsonce the cells have been found, they have the right to then be regarded at highermagnificatio
* Note - supplied cotton swabsand noodle towel need to be for sure discarded in the trash and not left lied onthe working table.
Why execute we have to Stain the Cells?
The cell has differentparts, and those that deserve to absorb stains or dyes are referred to as chromatic.Having absorbed the stain, these parts of the cell become much more visible underthe microscope and also can thus be easily distinguished from other parts ofthe very same cell.
Without stains, cell would appear to be practically transparent,making it challenging to identify its parts.
Methylene blue has actually a stringaffinity because that both DNA and RNA. Once it comes in contact with the two, a darkerstain is produced and also can be perceived under the microscope.
The nucleus at thecentral component of the cheek cell consists of DNA. When a drop of methylene blue isintroduced, the nucleus is stained, which provides it was standing out and also be clearlyseen under the microscope.
Although the whole cell appears light blue incolor, the nucleus in ~ the main part of the cell is lot darker, i m sorry allowsit to be identified.
On mounting the wet slide,the adhering to will it is in observed:
Large irregularly shaped cell with unique cell membranes.A distinct nucleus in ~ the main part of each individual cell(dark blue in color).
This is basic and funexperiment that will show kids the an easy structure that a cell and also its majorparts. For easy identification of the parts, the parental or teacher deserve to firstshow the children some samples the the cells in advance.This will assist themidentify various parts through ease.
Once this has actually been achieved, kids can moveon to the following stage of finding out the attributes of these various parts.
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** discover prepared microscope slides and also equipment to correctly perform microscope experiments.
See also: Epithelial Cells
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