A mutation, which might arise throughout replication and/or recombination, is a permanent change in the nucleotide succession of DNA. Damaged DNA have the right to be mutated one of two people by substitution, deletion or insertion of basic pairs. Mutations, for the most part, are harmless except when they lead to cell fatality or tumor formation. Since of the lethal potential of DNA mutations cells have advanced mechanisms for repairing damaged DNA.

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Types that Mutations

There are three species of DNA Mutations: base substitutions, deletions and insertions.

1. Basic Substitutions

Single basic substitutions space called point mutations, recall the suggest mutation Glu -----> Val which causes sickle-cell disease. Allude mutations space the many common type of mutation and also there room two types.

Transition: this occurs as soon as a purine is substituted with another purine or when a pyrimidine is substituted with an additional pyrimidine.

Transversion: as soon as a purine is substituted because that a pyrimidine or a pyrimidine replaces a purine.

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Point mutations that happen in DNA assignment encoding proteins room either silent, missense or nonsense.

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Silent: If abase substitution occurs in the third position the the codon over there is a great chance the a synonymous codon will be generated. Hence the amino acid sequence encoded by the gene is not changed and the mutation is said to it is in silent.

Missence: when base substitution results in the generation that a codon that specifies a different amino acid and hence leads to a different polypeptide sequence. Relying on the type of amino acid substitution the missense mutation is either conservative or nonconservative. For instance if the structure and properties that the substituted amino acid room very similar to the initial amino acid the mutation is stated to be conservative and will most likely have little effect top top the result proteins framework / function. If the substitution leads to one amino mountain with really different structure and also properties the mutation is nonconservative and also will most likely be deleterious (bad) for the result proteins structure / function (i.e. The sickle cell point mutation).

Nonsense: when a base substitution outcomes in a protect against codon at some point truncating translation and also most likely resulting in a nonfunctional protein.

2. Deletions

A deletion, bring about a frameshift, results when one or much more base bag are lost from the DNA (see number above). If one or two bases room deleted the translational framework is altered resulting in a garbled message and nonfunctional product. A deletion of three or more bases leave the reading frame intact. A deletion of one or an ext codons results in a protein missing one or more amino acids. This may be deleterious or not.

3. Insertions

The insertion of added base bag may result in frameshifts depending upon whether or no multiples of 3 base pairs are inserted. Combinations of insertions and also deletions causing a selection of outcomes are additionally possible.

Causes that Mutations

Errors in DNA Replication

On very, an extremely rare occasions DNA polymerase will certainly incorporate a noncomplementary base into the daughter strand. During the following round that replication the missincorporated basic would lead to a mutation. This, however, is very rare together the exonuclease attributes as a proofreading device recognizing mismatched base pairs and also excising them.

Errors in DNA Recombination

DNA frequently rearranges chin by a process called recombination i m sorry proceeds via a selection of mechanisms. Periodically DNA is lost during replication resulting in a mutation.

Chemical damages to DNA

Many chemistry mutagens, some exogenous, some man-made, part environmental, are qualified of damaging DNA. Countless chemotherapeutic drugs and intercalating agent drugs role by damaging DNA.

Radiation

Gamma rays, X-rays, even UV light can communicate with link in the cell generating free radicals which cause chemical damage to DNA.

DNA Repair

Damaged DNA have the right to be repaired by several different mechanisms.

Mismatch Repair

Sometimes DNA polymerase incorporates an untrue nucleotide throughout strand synthesis and the 3" to 5" editing and enhancing system, exonuclease, falls short to exactly it. These mismatches too as single base insertions and also deletions space repaired by the mismatch repair mechanism. Mismatch repair relies on a secondary signal within the DNA to distinguish between the parental strand and also daughter strand, which contains the replication error. Human cells posses a mismatch repair system comparable to the of E. Coli, which is described here. Methylation that the sequence GATC occurs on both strands at some point after DNA replication. Due to the fact that DNA replication is semi-conservative, the new daughter strand remains unmethylated for a really short duration of time following replication. This difference permits the mismatch repair mechanism to recognize which strand has the error. A protein, MutS recognizes and binds the mismatched base pair.

Another protein, MutL then binds to MutS and the partially methylated GATC succession is recognized and also bound through the endonuclease, MutH. The MutL/MutS facility then links with MutH which cuts the unmethylated DNA strand in ~ the GATC site. A DNA Helicase, MutU unwinds the DNA strand in the direction the the mismatch and also an exonuclease degrades the strand. DNA polymerase climate fills in the gap and ligase seals the nick. Defects in the mismatch fix genes uncovered in humans show up to be linked with the advance of hereditary colorectal cancer.

Nucleotide cut Repair (NER)

NER in person cells starts with the development of a complicated of proteins XPA, XPF, ERCC1, HSSB at the lesion on the DNA. The transcription variable TFIIH, which includes several proteins, then binding to the complex in an ATP dependence reaction and makes one incision. The resulting 29 nucleotide segment that damaged DNA is then unwound, the space is to fill (DNA polymerase) and the nick sealed (ligase).

Direct repair of Damaged DNA

Sometimes damage to a base have the right to be straight repaired by dedicated enzymes without having actually to excise the nucleotide.

Recombination Repair

This mechanism permits a cabinet to replicate previous the damage and also fix it later.

Regulation of damages Control

DNA repair is regulation in mammalian cell by a sensing system that detects DNA damage and activates a protein dubbed p53. P53 is a transcriptional regulatory element that controls the expression of part gene commodities that impact cell cycling, DNA replication and also DNA repair. Few of the attributes of p53, which are simply being determined, are: stimulation of the expression of gene encoding p21 and Gaad45. Lose of p53 duty can be deleterious, around 50% that all person cancers have a mutated p53 gene.

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The p21 protein binds and also inactivates a cell department kinase (CDK) which outcomes in cabinet cycle arrest. P21 additionally binds and also inactivates PCNA resulting in the inactivation of replication forks. The PCNA/Gaad45 facility participates in excision fix of damaged DNA.